|
KMID : 0371319930450040465
|
|
Journal of the Korean Surgical Society
1993 Volume.45 No. 4 p.465 ~ p.470
|
|
|
Study of Point Mutation at Codon 175 of p53 Gene in Breast Carcinoma by PCR
|
|
김영철
김종석/김선한/목영재/최상용/문홍영/구범환
|
|
|
Abstract
|
|
|
The p53 gene, which is located in the short arm of chromosome 17, has been a constant source of fascination since its discovery at 1979. Allelic deletion coupled with mutation of the remaining allele isa theoretical hallmark of functional
inactivation
of tumor suppressor gene, and mutation and/or deletion of p53 gene is to be the most frequently observed genetic changes in breast cancer related to a single gene. In the present report, we have attempted to determine a point mutation at codon
175
which
is known as one of four hat spots within highly conserved regions of the p53 gene where almost all of the reported mutations cluster. A 304-bp fragment of exon 5 of p53 gene in fresh tissue of breast carcinoma was amplified by polymerase chain
reaction(PCR), digested with Hha I restriction endonuclease, and electrophoresed. A point mutation was studied by observation of polymorphism with ethidium bromide staining under ultraviolet transillumination. The result revealed all of 14
carcinomas
had wild-type p53 gene.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|